Making RNA probes with T7 transcription
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mRNA sense & antisense probes
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In vitro T7 transcription of an RNA probe
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/f/f9/T7_primer_design_for_RNA_antisense_probes.png/500px-T7_primer_design_for_RNA_antisense_probes.png)
- designing a suitable primer combining the T7 promoter sequence and a sequence specific part
- attaching a T7 promoter via PCR
- in vitro transcription using T7 polymerase and the PCR product as a template