Northern blot

The RNA blot or Northern blot (named after the Southern blot for DNA fragments) is a molecular biology technique used to separate and identify pieces of RNA. RNA molecules are separated by mass on a gel, transferred (blotted) onto a cellulose or nylon membrane, and then labelled with complementary DNA or RNA molecules. These probes are either radioactive, typically ³²P, or contain labelled nucleotides, e.g. DIG-dNTPs, recognisable by antibodies. RNA molecules can be detected and roughly quantified via probe hybridisation.

See also

• BE.109:Systems engineering/Measuring DNA, RNA, protein
• RNA electrophoresis
• Endy:Northern Blot, 32P End-Labeled Probes
• RNA Extraction
• RNA