User:Moira M. Esson/Notebook/CHEM-581/2013/02/15: Difference between revisions
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# The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours. | # The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours. | ||
# Repeat this freeze-thaw cycle three times. | # Repeat this freeze-thaw cycle three times. | ||
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This procedure was adapted from [http://www.sciencedirect.com/science/article/pii/S0168365998000893] | |||
Preparation of Microspheres: | Preparation of Microspheres: | ||
*New calculations for the amount of DMSO/Rhodamine 6G to be added: | *New calculations for the amount of DMSO/Rhodamine 6G to be added: | ||
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*After the addition of dye/DMSO to the 110% Lamponite, the dye appeared to stick to very small spheres at the bottom of the beaker. Bright pink spheres immediately formed. This did not occur for the samples containing 110% NaMT. | *After the addition of dye/DMSO to the 110% Lamponite, the dye appeared to stick to very small spheres at the bottom of the beaker. Bright pink spheres immediately formed. This did not occur for the samples containing 110% NaMT. | ||
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==Fluorescence== | ==Fluorescence== | ||
*The six hydrogel samples that were allowed to soak in Rhodamine 6G were tested for the rate of diffusion of Rhodamine 6G from the samples. | *The six hydrogel samples that were allowed to soak in Rhodamine 6G were tested for the rate of diffusion of Rhodamine 6G from the samples. |
Revision as of 17:42, 17 February 2013
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Objectives
Microsphere Preparation
General Protocol:
For a 90:10 ratio: (25mL)(1μM)/(92μM)=0.272mL For a 50:50 ratio: (25mL)(1μM)/(165μM)=0.152mL
Fluorescence
General Protocol:
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