Malaria: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 6: | Line 6: | ||
**[[pCP08]] - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid | **[[pCP08]] - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid | ||
**[[pCP05]] - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid | **[[pCP05]] - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid | ||
**[[pCP03]] - Ad5 with NdeI site in fiber removed - hexon recombination plasmid | |||
all plasmids are AmpR | all plasmids are AmpR | ||
Revision as of 08:31, 30 August 2006
Live replicating adenovirus malaria vaccine
Plasmids
- Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
all plasmids are AmpR