Wittrup:Protocols: Difference between revisions
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===DNA=== | ===DNA=== | ||
[[Wittrup: Plasmid minipreps, restriction digestion]] | |||
[[Wittrup: Gel electrophoresis and band purification]] | |||
[[Wittrup: Site directed mutagenesis by QuikChange]] | |||
[[Wittrup: PCR]] | |||
===RNA=== | ===RNA=== | ||
Revision as of 00:40, 16 January 2006
Buffers/Equipment/Media
Assays
DNA
Wittrup: Plasmid minipreps, restriction digestion
Wittrup: Gel electrophoresis and band purification
Wittrup: Site directed mutagenesis by QuikChange
RNA
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup:ECL Western Blots and Phosphorimager
Wittrup:Protein concentration by ultrafiltration
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
