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| #Produce a [[SynBERC:COG/Report|report]] with evaluation of current assembly approaches and recommendations for going forward. | | #Produce a [[SynBERC:COG/Report|report]] with evaluation of current assembly approaches and recommendations for going forward. |
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| =COG:Meeting 1= | | =Meetings= |
| Agenda:
| | *[[SynBERC:COG/October 2007 Meeting]] |
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| ==What are the goals for COG?==
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| Proposed based on SynBERC meeting / conversations since:
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| #Stay up to date with current automated cloning research projects taking place in SynBERC labs
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| #Get the ball rolling for solving the automated cloning problem in the short term
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| ===Research Updates===
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| *1/2 the meeting?
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| *Brief updates from each of the groups? one longer presentation each month about one project?
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| **Something else?
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| ===Getting the ball rolling for solving automated cloning===
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| #Define the problem we want solved
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| #Specify the possible solutions
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| #Evaluate / Act on solutions
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| ====Define problem(s)====
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| *Combinatorial libraries of assembled parts (Wendall, UCSF iGEM team project)
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| *Automation of standard BioBrick Assembly (Registry)
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| *Automation of assembly independent of BBs (Synth companies?)
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| ====Specify Possible Solutions====
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| #Decentralized approach
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| #*We make cloning easier by using some automation combined with "normal" individual cloning approaches. For example, each lab buys a Qiacube to partially automate minipreps and restriction digests. Then ligations and transformations are done by hand at the bench.
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| #Centralized academic facility
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| #*We find the resources to start an assembly facility similar to how sequencing facilities are set up on campus
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| #Outsource to DNA synthesis company
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| #*We work with a DNA synthesis company so that they can offer commercial automated cloning services. How do we ensure that such a partnership wouldn't fall apart as soon as a big synthesis order comes along? Automated cloning may be too small a market and/or too large a problem for DNA synthesis companies to tackle right now?
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| ====Evaluate / Act on Solutions====
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| *Registry hires more staff to do process engineering
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| *Trial QIAcube / other "cheap" robots for distributed approach
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| *Figure out how big an assembly order iGEM + labs using parts could put together for a Synth company (George suggestion)
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| *Go talk to Synth companies see if they'll do this.
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| *Plot the cost of synth vs. assembly over time for particular length scales (drew's suggestion)
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| ==Meeting Frequency==
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| *Monthly?
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| *Any weekday's particularly bad for certain groups? (Weds at MIT)
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| ==Next steps==
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| =Communication= | | =Communication= |
Welcome to the Construction Optimization Group (COG)!
At the last SynBERC retreat SynBERCers decided that solving the challenge of automated DNA assembly/cloning would be a priority for the center. COG will be tasked with drafting a report for the upcoming NSF visit in March(?) that evaluates current assembly approaches and proposes a path forward. This page is just getting started, please add/edit!
Goals
- Maintain communication across labs on status of automated assembly projects.
- Produce a report with evaluation of current assembly approaches and recommendations for going forward.
Meetings
Communication
Members
Welcome to the Construction Optimization Group (COG)!
