Lidstrom:Sorvall Centrifuge

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Picture

Lidstrom Sorvoll centrifuge

Rotors

• Sorvall rotors
  • Rotors we have:
    • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
      • Tubes:

        

        SS-34 tubes

        • Can't be autoclaved.
          • Actually, Amanda might have autoclaved them before.
        • Can't write on body; can only write on cap. Sandy says stickers don't stick.
    • GS-3.
    • ____. Holds eppendorf (1.5mL) tubes
• For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.

There are stickers with maximum speeds. These were set by Katya, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.

Lidstrom sorvoll speed instructions

SS-34 rotor speeds and k factors

GS-34 rotor speeds and k factors

RCF explanation

Instructions

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

• Chill to 4^oC with SS-34 rotor inside
• Fill correct tubes
• Spin for ___ min at ___ RCF at ___^oC

Centrifugation Math & Basics

• Sigma summary:
  • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).

  • 

    Stoke's equation

  • 

    centrifugation speeds for applications