User:Allison K. Alix/Notebook/Thesis Research/2013/07/16
UV-Vis Analysis/Silica Coating Pt. 2 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
To Do
Silica Coating Procedure1) Prepare the following solutions:
3) Centrifuge solutions at 4000 rpm for one hour 4) Re-suspend particles in PBS to a final concentration of 5nM? DNA/AuNP Data AnalysisSee File:2013 0715 AuNP-DNA.xlsx for AuNP/DNA spectra as well as absorbance values and molar extinction coefficients used to calculate concentrations of each in final solutions. Calculating the surface area of 1 AuNP Surface Area of a sphere = 4πr2, r = 5nm 4π(5x10-9m)2 = 3.14 x 10-16m2 = 314nm2 Calculating the # of AuNP in 240.7μL 29.67nM solution 29.67 x 10-9mol/L x 240 x 10-6L x 6.02x1023 AuNP/mol = 4.299 x 1012 AuNP Calculating the Total Surface Area Available for DNA Attachment 4.299 x 1012 AuNP x 3.14x10-16m2/1 AuNP = 0.001349886 m2 Calculating the Number of Moles of DNA Available to Attach 2.225x10-6moles/L x 240.7x10-6L = 5.3555575 x 10-10 moles DNA Calculating oligonucleotide density on each particle 5.3555575x10-10moles DNA/0.001349886m2 AuNP = 3.967x10-7 moles DNA/m2 AuNP (OR 39.67pmol DNA/cm2 AuNP) Calculating the percentage of DNA hybridized in each sample
Sample A1 moles DNA = (1.827x10-7M x 350 x 10-6L) = 6.40x10-11 moles % hybridized = (6.40x10-11/5.3555575 x 10-10 moles DNA)x100% = 11.95% Sample B moles DNA = 4.44x10-8M x 500 x 10-6L = 2.22x10-11 moles % hybridized = (2.22x10-11 moles/5.3555575 x 10-10 moles DNA)x100% = 4.14% Sample A2 moles DNA = 1.42x10-10 % hybridized = (1.42x10-10/5.3555575 x 10-10) = 26.5%
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