20.109(S13): Primer design summary

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20.109(S13): Laboratory Fundamentals of Biological Engineering

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You will write a brief memo about your study of diagnostic primers for identifying microsporidia.

The target audience is an advisor for whom you are preparing a relatively informal (but accurate!) report.


Please email your completed summary to 20109 DOT submit AT gmail DOT com, with filename Firstinitial_Lastname_LabSection_Mod1.doc (for example, R_Reif_TR_Mod2.doc).

This assignment is due by 11 am on March 19th (Tuesday) or March 20th (Wednesday), according to which day you have lab.

Content Guidelines

Two primary sections -- one on design and one on results -- will make up the memo.


Here you should include the following:

  • A short (1-2 paragraph) introduction motivating your design challenge and explaining your design choices.
  • A table comparing the properties of the V1-PMP2 primer set and your primer set.

The text related to your specific design choices should explicitly refer to the table.


Here you should include the following:

  • A short (1-2 paragraph) description of your findings.
  • A figure depicting your raw PCR results (as seen on a gel).
  • Optionally, a figure or table depicting the PCR results in some processed form.

The figure should include a complete caption as for a publication.