20.109(F07):Module 1:RefactorM13
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All parts are specified at the Registry of Standard Biological Parts. Refine the parts there as well as here, adding your comments to the area below the table.
genetic element | promoter | RBS | coding |
---|---|---|---|
start synthesis with | |||
gII | BBa_M13102 (need 5' UTR?) |
BBa_M13502 | BBa_M13002' (modified to remove gene 10 promoter) |
gX | BBa_M13110 (need 5' UTR?) |
BBa_M13510 | BBa_M13010' (modified to remove gene 5 promoter) |
gV | BBa_M13105 (need 5' UTR?) |
BBa_M13505 | BBa_M13005 |
gVII | BBa_M13507 | BBa_M13007' (modified to remove overlap with gene 9 dwnstm) | |
gIX | BBa_M13509 | BBa_M13009' (modified to remove overlap with gene 8 dwnstm) | |
gVIII | BBa_M13108 (need 5' UTR?) |
BBa_M13508 | BBa_M13008 |
Transcriptional terminator (if M13K07 part, then need to modify to remove gene 3 promoter) | |||
gIII | BBa_M13103 | BBa_M13503 | BBa_M13003' (modified to remove gene 6 promoter, change GTG start?) |
gVI | BBa_M13106 | BBa_M13506 | BBa_M13006' (modified to remove gene 1 promoter) |
gI | BBa_M13101 | BBa_M13501 | BBa_M13001' (modified to remove gene 11 RBS, gene 4 promoter, RBS, start) |
gXI | BBa_M13511 | BBa_M13011' (modified to remove gene 4 promoter, RBS, start) | |
gIV | BBa_M13104 (need 5' UTR?) |
BBa_M13504 | BBa_M13004' |
M13K07 ori/KanR/p15a ori | |||
end synthesis |
Note: modified parts codon varied to remove direct repeats.