User:Wesley J. Houston/Notebook/MAP/2013/03/11

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3/11/13 Grow liquid cultures of KAH182 to repeat ligation. Retry ligation with lower concentration plasmids. > Ligations   1 2 Insert DNA (KAH182) 4.0 --- Vector DNA (KAH201) 0.65 0.65 2x lgn buf (Roche) 5.0 5.0 T4 ligase (NEB) 1.0 1.0 dH2O 0 3.5   10 μL 10 μL Follow quick transformation protocol Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar Ligation Plate results (lig : neg crtl) 03/11/13 1. KAH201 (S/P)/3403, 25 ng + KAH182 (X/P)/1675, 26 ng 0 2. KAH201 (S/P)/3403, 25 ng 0