User:Wesley J. Houston/Notebook/MAP/2013/03/08

Project name

Main project page Previous entry      Next entry

3/8/13 Ligation was repeated. Attempted to grow more liquid cultures of ligated plasmids. Attempted to confirm ligation by streaking the colonies on an agar plate. All attempts proved unsuccessful. > Ligations   1 2 Insert DNA (KAH182) 3.0 --- Vector DNA (KAH201) 0.5 0.5 2x lgn buf (Roche) 5.0 5.0 T4 ligase (NEB) 1.0 1.0 dH2O 0.5 3.5   10 μL 10 μL Follow quick transformation protocol Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar Ligation Plate results (lig : neg crtl) 03/08/13 1. KAH201 (S/P)/3403, 25 ng + KAH182 (X/P)/1675, 26 ng 0 2. KAH201 (S/P)/3403, 25 ng 0 No colonies were present in the ligation plate.