User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/24

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DNA Validation Digestion 10 μL DNA 2 μL 10X NEBuffer 4 --> 52 μL 1 μL NdeI --> 26 μL 7 μL MilliQ --> 182 μL 37°C for 1 hr. Expected Patterns Good: 6040, 1416bp Empty: 6645bp Gel 0.8% Agarose w/ EtBr All colonies gave correct pattern except for 2 and 23