User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/23

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Colony Screen Poured off 1-1.5 mL of each colony into an eppendorf tube. Added 15 μL of 0.1M IPTG to each 4 mL culture. Shook at 37°C for 2.5 hrs. Screened each culture for green fluorescence High background autofluorescence No colonies stood out