|
Objective
- Perform Gel Electrophoresis on PCR products prepared on 9/20/2011 to determine whether or not GFP was successfully mutated to contain a cystein residue after the enterkinase cleavage site.
Description
- To produce the gel, the following procedure was made.
- 0.25g of Agarose (0.01g/ml).
- Agarose was mixed with 25ml of 1x TAE buffer.
- The mixture was placed into a microwave for 40 seconds.
- The hot mixture was poured into tray to form gel sheet and was left to cool.
- Approximately 25ul of pink wax was removed from DNA sample.
- 1ul of 6x loading dye was placed on parafilm.
- The loading dye was mixed with 5ul of DNA.
- All of the DNA samples were placed in gel from wells 1 to 6.
- The Ladder was in well 1.
- The gel was run at 80V for 40 minutes.
- The gel was carefully removed and placed in ethidium bromide and TAE buffer solution.
- The gel was washed with TAE buffer for five minutes.
Data
- Observation of the gel indicated the presence of faint bands at the right side of the 2kb band in the ladder.
- From top to bottom, the band sizes for the ladder are 10, 8, 6, 5, 4, 3, 2, 1.5, 1, and 0.5 kilobases.
Notes
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
.
|
Project name
Main project page
Previous entry Next entry
