User:Nader A. Khamis/Notebook/Experimental Biological Chemistry AU Fall 2011/2011/09/20
|Project name|| Main project page|
Previous entry Next entry
Forward Primer: 5' - CGA CGA TGA CGA TAA GCG ATG GGG ATC CGA ATT CGC - 3'
Reverse Primer: 5' - GCG AAT TCG GAT CCC CAT CGC TTA TCG TCA TCG TCG - 3'
Melting Temperature of Theoratical Primers (Tm) = 79.1°C Percent of GC content= 56%.
Forward primer used was:5' GAC GAT GAC GAT AAG GAT CGA TGG GGA TCC GAA 3'
Reverse primer used was: 5' TTC GGA TCC CCA CCA TCG ATC CTT ATC GTC ATC GTC 3'
Melting Temperature of actual primers = 64.6°C Percent of GC content = 51.5%
5 uL 10x reaction buffer
42 uL dH2O
1 uL dsDNA template (50 ng/uL)
1.25 uL Primer 1
1.25 uL Primer 2
1 uL dNTP mix (10 mM)
The final cycle was: 4°C for 24 hours.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. .