User:Meng Xiao He/Notebook/fall08/2008/11/11

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Sequencing results M13R primer used TOPO A is cco TOPO B is empty TOPO C is Duo pUC19 + Duo does seem to be complete flank (alignment goes past middle site) pER21 (taken from original glycerol stock strain) does NOT seem to contain the M13 reverse sequencing site Transformation failure Electroporated C1 and B2 DNA into wt MR-1> no colonies after 2 days on LB-GM selection