User:Meng Xiao He/Notebook/fall08/2008/11/13

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pER21+Duo ligation

Ligated the Duo fragment from pUC19+Duo into new, old, and concentrated pER21 fragments

  • All vectors dephos'ed
  • Used Takara ligase
  • 10 ul into 100 ul chem comp cells

B2, C1

By PCR B2 is cbb3 & C1 is cco. The product was smeary, so not sure if they contain the full flank products, but they're not empty and not other things.

Primer order

Ordered sequencing primers to look at KO plasmids. 2 for each flank; may not be sufficient for all cases to get entire read.


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