User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/29

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Trying to handle as many things as possible!!!

  • Today, I checked the gel of my restrictions and as I observed the correct size in a reasonable concentration, I ligated them using the following reaction for a total of 20μl:

-H2O --------------------> 7μL

-Buffer ligase ----------> 4μL

-Vector (plasmid) -----> 2μL

-Insert (luciferase) ---> 6μL

-Ligase -----------------> 1μL

  • I left the ligations at 16°C ON.

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