There it goes!
- Today, I checked that the restrictions had the appropriate size, which can be observed next:
- The lanes indicate that ligations were empty, so I will need to re-ligate them :(.
- To fix this problem, I was planning on ligating using a type of three antibiotic assembly. However, when I ran the gel to check the insert and vector relation, I saw nothing where the insert (luciferase) should be :(.
- Luckily, I found a mutated luciferase stock, so I will leave a restriction for a total of 30μl as follows ON at 37°C.
- H2O -------------> 13
- Buffer 3 ------> 3μL
- BSA ------------> 1μL
- DNA ------------> 10μL
- XBA I ---------> 1.5μL
- PST I ----------> 1.5μL
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WiFi coli: A communicolight system
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