User:Khoff

I am a member of the Smolke Lab at Caltech. My research is directed toward developing in vivo biosensors with the ability to detect fluctuations in metabolite and protein concentrations as well as post-translational modifications.

Research Interests

My background is in the study of protein structure-function relationships. Through this work I have become interested in the changes in protein modifications and metabolite flux that occur during the cell cycle. I am particularly interested in the way post-translational modifications and small molecules influence epigenetic control of transcription.

Education

• 2005-present Postdoctoral Scholar, Laboratory of Dr. Christina D. Smolke, Caltech
  
• 2002-2005 HHMI Post-Doctoral Fellow, Laboratory of Dr. Cynthia Wolberger, The Johns Hopkins School of Medicine.
  
• 2002 Ph.D. Biophysics, Laboratory of Dr. Larry E. Vickery, University of California, Irvine.
  
• 1996 B. A. Biology/Chemistry Minor, California State University, Fullerton
  

Publications

1. Hoff KG, Goodlitt R, Li R, Smolke CD, and Silberg JJ. Fluorescence detection of a protein-bound 2Fe2S cluster. Chembiochem 2009 Mar 2; 10(4) 667-70. doi:10.1002/cbic.200800747 pmid:19184988. PubMed HubMed PubGet [hoff15]
2. Bayer TS, Hoff KG, Beisel CL, Lee JJ, and Smolke CD. Synthetic control of a fitness tradeoff in yeast nitrogen metabolism. J Biol Eng 2009 Jan 2; 3 1. doi:10.1186/1754-1611-3-1 pmid:19118500. PubMed HubMed PubGet [hoff14]
3. Beisel CL, Bayer TS, Hoff KG, and Smolke CD. Model-guided design of ligand-regulated RNAi for programmable control of gene expression. Mol Syst Biol 2008; 4 224. doi:10.1038/msb.2008.62 pmid:18956013. PubMed HubMed PubGet [hoff13]
4. Hawse WF, Hoff KG, Fatkins DG, Daines A, Zubkova OV, Schramm VL, Zheng W, and Wolberger C. Structural insights into intermediate steps in the Sir2 deacetylation reaction. Structure 2008 Sep 10; 16(9) 1368-77. doi:10.1016/j.str.2008.05.015 pmid:18786399. PubMed HubMed PubGet [hoff12]
5. Hoff KG, Avalos JL, Sens K, and Wolberger C. Insights into the sirtuin mechanism from ternary complexes containing NAD+ and acetylated peptide. Structure 2006 Aug; 14(8) 1231-40. doi:10.1016/j.str.2006.06.006 pmid:16905097. PubMed HubMed PubGet [hoff11]

• Previewed in Smith BC and Denu JM. Sirtuins caught in the act. Structure. 2006 Aug;14(8):1207-8.

6. Hoff KG and Wolberger C. Getting a grip on O-acetyl-ADP-ribose. Nat Struct Mol Biol 2005 Jul; 12(7) 560-1. doi:10.1038/nsmb0705-560 pmid:15999106. PubMed HubMed PubGet [hoff1]
7. Silberg JJ, Tapley TL, Hoff KG, and Vickery LE. Regulation of the HscA ATPase reaction cycle by the co-chaperone HscB and the iron-sulfur cluster assembly protein IscU. J Biol Chem 2004 Dec 24; 279(52) 53924-31. doi:10.1074/jbc.M410117200 pmid:15485839. PubMed HubMed PubGet [hoff2]
8. Hoff KG, Cupp-Vickery JR, and Vickery LE. Contributions of the LPPVK motif of the iron-sulfur template protein IscU to interactions with the Hsc66-Hsc20 chaperone system. J Biol Chem 2003 Sep 26; 278(39) 37582-9. doi:10.1074/jbc.M305292200 pmid:12871959. PubMed HubMed PubGet [hoff3]
9. Hoff KG, Ta DT, Tapley TL, Silberg JJ, and Vickery LE. Hsc66 substrate specificity is directed toward a discrete region of the iron-sulfur cluster template protein IscU. J Biol Chem 2002 Jul 26; 277(30) 27353-9. doi:10.1074/jbc.M202814200 pmid:11994302. PubMed HubMed PubGet [hoff4]
10. Urbina HD, Silberg JJ, Hoff KG, and Vickery LE. Transfer of sulfur from IscS to IscU during Fe/S cluster assembly. J Biol Chem 2001 Nov 30; 276(48) 44521-6. doi:10.1074/jbc.M106907200 pmid:11577100. PubMed HubMed PubGet [hoff5]
11. Voisine C, Cheng YC, Ohlson M, Schilke B, Hoff K, Beinert H, Marszalek J, and Craig EA. Jac1, a mitochondrial J-type chaperone, is involved in the biogenesis of Fe/S clusters in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 2001 Feb 13; 98(4) 1483-8. doi:10.1073/pnas.98.4.1483 pmid:11171977. PubMed HubMed PubGet [hoff6]
12. Silberg JJ, Hoff KG, Tapley TL, and Vickery LE. The Fe/S assembly protein IscU behaves as a substrate for the molecular chaperone Hsc66 from Escherichia coli. J Biol Chem 2001 Jan 19; 276(3) 1696-700. doi:10.1074/jbc.M009542200 pmid:11053447. PubMed HubMed PubGet [hoff7]
13. Hoff KG, Silberg JJ, and Vickery LE. Interaction of the iron-sulfur cluster assembly protein IscU with the Hsc66/Hsc20 molecular chaperone system of Escherichia coli. Proc Natl Acad Sci U S A 2000 Jul 5; 97(14) 7790-5. doi:10.1073/pnas.130201997 pmid:10869428. PubMed HubMed PubGet [hoff8]
14. Garland SA, Hoff K, Vickery LE, and Culotta VC. Saccharomyces cerevisiae ISU1 and ISU2: members of a well-conserved gene family for iron-sulfur cluster assembly. J Mol Biol 1999 Dec 10; 294(4) 897-907. doi:10.1006/jmbi.1999.3294 pmid:10588895. PubMed HubMed PubGet [hoff9]
15. Silberg JJ, Hoff KG, and Vickery LE. The Hsc66-Hsc20 chaperone system in Escherichia coli: chaperone activity and interactions with the DnaK-DnaJ-grpE system. J Bacteriol 1998 Dec; 180(24) 6617-24. pmid:9852006. PubMed HubMed PubGet [hoff10]

Protocols and Recipes

Protocols

Recipes and other stuff

• Lab coffee

• Lab drinks