User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/12

Purpose

• Start DH5α-T1 cell growth on petri dish for future use.

Procedure

• Plates plated from 2013/02/06 were transferred to fridge at 20°C due to neglect.
• In order to ensure consistent cell growth for each experiment, a new dish was plated.
• In a sterile environment, an autoclaved wooden stick was used to dip into the DH5α-T1 disk made on 2013/02/06 and strikes on a new LB+agar plate.
• The dish was placed in -37°C for over night (16 hours).