User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/13

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Purpose

  • Make starter culture of DH5α-T1 cells in order to start pilot study.

Procedure

  • DH5α-T1 plate was taken out this morning and placed in 20°C refrigerator
  • In a sterile environment, 3mL of autoclaved LB was transferred into a 15mL falcon tube.
  • With an autoclaved wooden stick, a cell colony from cell plate was obtained and dipped into the 3mL LB.
  • The cell containing LB media was then placed into an orbital shaker at 230rpm for 16 hours.



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