User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/01

AU CHEM-570 Lab Prep

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Objective Determine whether Ins_His_after_CBD and Rins_His_after_CBD primers will appropriately match sequence around C-term of CBD in pMXB10 plasmid If acceptable, QuikChange of pMXB10 plasmid with above His-tag primers Transform test ligations from 7/28 Bench work Verified complementarity of His-tag primers QuikChange protocol with pMXB10 Diluted new stock from 200μg/mL to 10μg/mL Followed basic QuikChange protocol with 8 minute elongation step (plasmid ~7.8kbs) Transformation of: New ligation of pTXB1 (digest by EcoRI, BamHI, arctic phosphatase) 7/28 New ligation of pTXB1 (digest by EcoRI, BamHI) 7/28 pTXB1 (digest by EcoRI, BamHI, arctic phosphatase) 7/26 pTXB1 (digest by EcoRI, BamHI) 7/26 Results Add results here...