User:Jose Fabricio Lopez/Notebook/Logbook/2011/09/11

iGEM 2011

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Goals Repeat Backbones for these PCRs PCR 3 PCRs for TerB0015-Backbone Oligos for 1st Terminator Reagents μL MegaMix H2O 24.5 74 5X Phusion Buffer HF 10 30.5 10mM dNTPs 1 each 3.1 each Primer F 5 15.2 Primer R 5 15.2 DNA 1 3 Phusion .5 NA 3 PCR for TerB0015-Backbone Oligos for 2nd Terminator Reagents μL MegaMix H2O 24.5 74 5X Phusion Buffer HF 10 30.5 10mM dNTPs 1 each 3.1 each Primer F 5 15.2 Primer R 5 15.2 DNA 1 3 Phusion .5 NA Cycles Gel instruct. Gel 98°C 30s 98°C 10s 30Cy 60°C 30S 30Cy 72°C 1:50 30Cy 72°C 10min 4°C Lane2 Ladder Lane3-5 Ter1-Backbone 1 Lane6-8 Ter2-Backbone 2