User:Jose Fabricio Lopez/Notebook/Logbook/2011/09/13

iGEM 2011

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Goal Repeat PCR PCR Using .1X dilution of extraction Reagent FinalReact MasterMix Ter1 MasterMix Ter2 H2O 24.5 50 50 5X Phusion Buffer HF 10 20.5 20.5 10mM dNTPs 1 each 2.1 each 2.1 each Primer F 5 10.2 10.2 Primer R 5 10.2 10.2 DNA 1 2 2 Phusion .5 NA NA Cycles GelInst Gel 98°C 10sec 30 Rep 98°C 15sec 55°C&59°C 30sec 72°C 1:50 min 72°C 10min 4°C αΩ Lane1 Ladder Lane2 Ter1 55°C Lane2 Ter1 59°C Lane2 Ter2 55°C Lane2 Ter2 59°C