User:Jose Fabricio Lopez/Notebook/Logbook/2011/09/13

From OpenWetWare

Jump to: navigation, search
iGEM 2011 Main project page
Previous entry      Next entry

Goal


PCR

Using .1X dilution of extraction

Reagent FinalReact MasterMix Ter1 MasterMix Ter2
H2O 24.5 50 50
5X Phusion Buffer HF 10 20.5 20.5
10mM dNTPs 1 each 2.1 each 2.1 each
Primer F 5 10.2 10.2
Primer R 5 10.2 10.2
DNA 1 2 2
Phusion .5 NA NA
Cycles GelInst Gel

98°C 10sec


30 Rep

98°C 15sec

55°C&59°C 30sec

72°C 1:50 min

72°C 10min


4°C αΩ

  • Lane1 Ladder
  • Lane2 Ter1 55°C
  • Lane2 Ter1 59°C
  • Lane2 Ter2 55°C
  • Lane2 Ter2 59°C



Personal tools