Objectives
- Plasmid restriction, with SpeI.
Restriction
- I need a 2nd restriction for |pUX19-XkR5 plasmid, because the restriction of yesterday shows an unexpected pattern of 3 bands.
- I cut with SpeI because originally pUX19 has 2 sites, those sites flank insert of RepC-antisenseRNA, with that I expect 2 band:
- Larger band with 3.9kb
- Smaller band with 1.4KB(This is because insert for XKR5 plasmid has ~1.4Kb)
Reactives
|
Solutions
|
SpeI
|
2μL
|
Buffer 4 NEB
|
2μL
|
BSA10x
|
2μL
|
pUX19Extraction
|
5μL
|
H2O
|
9μL
|
Total
|
20μL
|
- Lane 1= Ruler
- Lane 2= pUX19XKR5 cut.
|