User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/06

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Objectives

Transfromation

  • Success.

Plasmid purification

  • Performed with miniprep from Miguel´s Lab.
  • Cell grown up by 6 hours in LB with Km 30. 3 tubes with 5 mL and control.
    • 6 tubes with 2.5 mL each one. 6 tubes were mixed in one tube with 60μL TE-RNAse.
  • Lane 1 Ruler
  • Lane 2 Plasmid

Gel pUX19... extraction

The purpose of this gel is to see concentration to cut plasmid and see if that is the correct plasmid.



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