User:Ilya/Yeast/Mating pheromone response pathway
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Contents |
[edit] Components
[edit] Miscellaneous
- Crosstalk in the pathways is eliminated by formation of pathway-specific complexes (Ste5:Ste11:Ste7:Fus3, Pbs2:Ste11:Hog1)
- In S. cerevisiae, meiosis is initiated only by diploid cells deprived of glucose and nitrogen and grown in the presence of a nonfermentable carbon source [1]
[edit] Pheromone processing genes
- STE14
- carries out C-terminal methylation of prenylated proteins including a-factor, Ras1p, and Ras2p
- encodes the prenylcysteine-dependent carboxyl methyltransferase that mediates methylation, the final step in modification of CAAX proteins; Ste14p is also membrane associated [2]
- STE13
- Dipeptidyl aminopeptidase A involved in maturation of a-factor
- AXL1
- Protease involved in proteolytic step of a-factor N-terminal processing
- STE23
- RAM1, RAM2
- Required for a-factor prenylation
- STE24
- RCE1
- KEX2
- KEX1
- STE23
[edit] Other genes
These genes are part of the mating machinery in yeast but not necessary part of the mating pheromone response pathway.
- Swe1 - protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity
- Ras1 - pombe homolog interacts with MAPKKK
- SAG1 (α-agglutinin) - provides tight cell-cell adhesion during mating in S. cerevisiae.
- FUS1 - protein with SH3 domain required for cell fusion during mating, located at the tip of the mating projection
- BNI1/YNL271C (Saccharomyces cerevisiae)
- Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
[edit] References
- Pathway architecture overview
- Tzung KW, Williams RM, Scherer S, Federspiel N, Jones T, Hansen N, Bivolarevic V, Huizar L, Komp C, Surzycki R, Tamse R, Davis RW, and Agabian N. Genomic evidence for a complete sexual cycle in Candida albicans. Proc Natl Acad Sci U S A 2001 Mar 13; 98(6) 3249-53. doi:10.1073/pnas.061628798 pmid:11248064.
- Ashby MN, Errada PR, Boyartchuk VL, and Rine J. Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: carboxyl methyltransferase. Yeast 1993 Aug; 9(8) 907-13. doi:10.1002/yea.320090810 pmid:8212897.
- Kofahl B and Klipp E. Modelling the dynamics of the yeast pheromone pathway. Yeast 2004 Jul 30; 21(10) 831-50. doi:10.1002/yea.1122 pmid:15300679.
- Banuett F. Signalling in the yeasts: an informational cascade with links to the filamentous fungi. Microbiol Mol Biol Rev 1998 Jun; 62(2) 249-74. pmid:9618441.
- Bardwell L. A walk-through of the yeast mating pheromone response pathway. Peptides 2004 Sep; 25(9) 1465-76. doi:10.1016/j.peptides.2003.10.022 pmid:15374648.
