User:Beatriz Gimenez De C./Notebook/571/2014/09/23

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tasks Retreive electrophoresis samples prepared and refrigerated las week Preparations : 50 μL of 1g/L Lysozyme stock solution and dilute to 5 mL with 50 mM Glycine stock solution in volumetric flask. Take 10 μL of diluted solution (Lysozyme stock and glycine solution) and 10 μL of running buffer (prepared by Dr. Fox) and add to 1.5 mL centrifuge tube, vortex for 5-10 seconds. 50 μL of 30:1 [Au]:[Lysozyme] colloid solution, dilute + take 10μL of diluted solution and 10μL of running buffer solution and add to 1.5 mL centrifuge tube + vortex for 5-10 seconds 50 μL of 30:1 [Au]:[BSA] colloid solution, dilute + take 10 μL of diluted solution and 10 μL running buffer and add to centrifuge tube. + vortex for 5-10 seconds 50 μL of 1g/L BSA stock solution (prepared by Dr. Fox), dilute + take 10 μL of diluted solution + 10 μL of running buffer + add to 1.5 mL centrifuge tube + vortex for 5-10 seconds Load 15 μL of samples (stated above, after 1 week refrigerated storage) Well 3: Lysozyme Well 5: BSA Colloid Well 7: Lysozyme Colloid Well 9: Soy Well 11: All Blue Protein Standard (provided by Bio-Rad) Run Electrophoresis for 30 minutes at 200 V Staining Gel process (repeated twice) -> Place gel in Fixative Solution (40% methanol, 10% acetic acid, 50% water) during 30 minutes + Place gel in Stain Solution duringr 1 hour + Place gel in Destain Solution during 15 minutes note had to leave early because indisposed Alicia's or Khyra's notebook for further detail