User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/24

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Contents

Nuclear Region Adenita Culture 454 Amplification

Reaction mixture conc. 1x x61 ' lane Template Primer mix
Template??31831Harvard A2 (#10)B10
ExTaq buffer10x1612C10
dNTP mix2.5 mM each0.848.83D10
Primer mix20 uM each14E10
ExTaq5 U/ul0.053.055F10
Milli Q4.15253.156Harvard A3 (#11)B11
10 ul7C11
8D11
PCR cycle9E11
98C10 sec10F11
60C1 min11Ithaca 1 B1 (#17)B5
x3512C5
13D5
14E5
15F5
16Ithaca 1 B3 (#18)B6
17C6
18D6
19E6
20F6
21Ithaca 1 B5 (#19)B7
22C7
23D7
24E7
25F7
26Ithaca 1 C1 (#20)B8
27C8
28D8
29E8
30F8
31Ithaca 1 D1 (#21)B9
32C9
33D9
34E9
35F9
36Ithaca 2 A3 (#27)B3
37C3
38D3
39E3
40F3
41Ithaca 2 A4 (#28)B4
42C4
43D4
44E4
45F4
46Ithaca 2 A5 (#29)B5
47C5
48D5
49E5
50F5
51MilliQ (negative control)B10
52C10
53D10
54E10
55F10
56AV_HMW (20ug)B10
57C10
58D10
59E10
60F10

load 2.0 ul of sample

Image:0097.jpg


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