User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/26

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Contents

Nuclear Region Adenita Culture 454 Amplification

Re-Amp of 19May10 Re-Extracted Cultures 31, 32, 36, 37, 38 & 39

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water4.1520.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene315
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
131 InstageneB72138 InstageneB2
231 InstageneC72238 InstageneC2
331 InstageneD72338 InstageneD2
431 InstageneE72438 InstageneE2
531 InstageneF72538 InstageneF2
632 InstageneB82639 InstageneB3
732 InstageneC82739 InstageneC3
832 InstageneD82839 InstageneD3
932 InstageneE82939 InstageneE3
1032 InstageneF83039 InstageneF3
1136 InstageneB1231Neg ControlB7
1236 InstageneC1232Neg ControlC7
1336 InstageneD1233Neg ControlD7
1436 InstageneE1234Neg ControlE7
1536 InstageneF1235Neg ControlF7
1637 InstageneB136Pos ControlB7
1737 InstageneC137Pos ControlC7
1837 InstageneD138Pos ControlD7
1937 InstageneE139Pos ControlE7
2037 InstageneF140Pos ControlF7
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:26May10A.jpg

Nuclear Region Adenita Culture 454 Amplification

Re-Amp of 26May10 Re-Extracted Cultures 33, 34, 35, 40, 41, 42, 43

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water4.1520.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene315
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
133 InstageneB92141 InstageneB5
233 InstageneC92241 InstageneC5
333 InstageneD92341 InstageneD5
433 InstageneE92441 InstageneE5
533 InstageneF92541 InstageneF5
634 InstageneB102642 InstageneB6
734 InstageneC102742 InstageneC6
834 InstageneD102842 InstageneD6
934 InstageneE102942 InstageneE6
1034 InstageneF103042 InstageneF6
1135 InstageneB1131Neg ControlB9
1235 InstageneC1132Neg ControlC9
1335 InstageneD1133Neg ControlD9
1435 InstageneE1134Neg ControlE9
1535 InstageneF1135Neg ControlF9
1640 InstageneB436Pos ControlB9
1740 InstageneC437Pos ControlC9
1840 InstageneD438Pos ControlD9
1940 InstageneE439Pos ControlE9
2040 InstageneF440Pos ControlF9
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:26May10B.jpg

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