Start here: PMID: 16085812
and also consider this one: PMID: 19060885
Your goal is to make a new flagellar display system, possibly a circularly permuted flagellar protein, or a secreted adapter fusion protein. We want a system that is N-terminal or C-terminal (not in a loop). The most likely candidate is FliD, but also consider FliC and other E. coli K12 flagellar proteins, etpA and it's Yersinia pseudotuberculosis homolog CP000720.
You may also need to use a fli gene knockout. Once you know what you are going to make, we'll obtain the clone from the Keio collection.
Questions you need to ask when reading:
- Is there a reference showing your target protein can be knocked out and re-complemented resulting in effective chemotaxis?
- Is there structural information sufficient to guide the construction of a circularly-permuted variant?
In the end, you'll be making 1 or more Displayer parts. You'd probably want to try both N and C terminal display types. Follow these instructions about where to put your part based on the type: