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- IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/21 (section Ligation/Transformation of Mira/Brazz+StrepII)ladder 2. Mira N1 XbaI/PstI 3. Mira N2 XbaI/PstI 4. Brazz C1 XbaI/PstI 5. Brazz C2 XbaI/PstI 6. 1kb plus ladder 7. Mira N1 undigested 8. Mira N2 undigested...5 KB (0 words) - 19:18, 26 September 2017
- IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/14 (section DTL of StrepII+Mira/Brazz with STOP codon)E/S STOP + V0120 E/X 1kb Plus Ladder Gel Purification Specs Mira N StrepII E/S: 3.0 ng/μL Mira C StrepII E/S: 2.8 ng/μL Brazz N StrepII E/S: 3.4 ng/μL Brazz...7 KB (0 words) - 19:14, 26 September 2017
- IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/06 (section Transformation of Mira/Brazz StrepII constucts into E45)cells were plated on LB+AMP and left to grow O/N Transformations: Mira N1 Mira N2 Mira C1 Mira C2 Brazz N1 Brazz N2 Brazz C1 Brazz C2 pACT2 1μL Bam 1μL Xho...3 KB (0 words) - 19:27, 26 September 2017
- IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/27 (section Ligation of Mira/Brazz + StrepII to STOP + V0120)5 KB (0 words) - 19:21, 26 September 2017
- IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/02 (section DTL of Mira/Brazz+StrepII+Stop into V24)plated on LB+AMP plates and left in 37°C incubator overnight Ladder Mira N SS Mira C SS Brazz N SS Brazz C SS V24 N/S B21 X/P Ladder Gel bands indicated...4 KB (0 words) - 19:25, 26 September 2017
- IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/19 (section DTL of Mira/Brazz+StrepII into STOP+V0120)Ligation Reactions Mira N + Stop Mira C + Stop Brazz N + Stop Brazz C + Stop Stop only Control diH2O 3 3...6 KB (0 words) - 19:17, 26 September 2017
- 3 KB (0 words) - 19:20, 26 September 2017
- ng/μL Confirmation Digest of Mira/Brazz + YFP + NOSt + STOP in V24 Expression Vector Ladder Mira N1 Mira N2 Mira C1 Mira C2 Brazz N1 Brazz N2 Brazz C1...7 KB (0 words) - 19:15, 26 September 2017
- S/P Mira+YFP N1 Mira+YFP N2 Mira+YFP C1 Mira+YFP C2 Brazz+YFP N1 Brazz+YFP N2 Brazz+YFP C1 Brazz+YFP C2 Ladder It appears that the digestion of Mira+YFP...5 KB (0 words) - 19:11, 26 September 2017
- algorithm producing superior contigs. Requires phrap and patched ReAligner MIRA latest version 2.9.25 enables true hybrid sequence assembly (454 data [GS20...4 KB (436 words) - 15:34, 12 February 2010
- confirm: failed Digested Mira/Brazz+StrepII constructs EcoRI/SpeI, STOP+V0120 EcoRI/XbaI Digestion Reactions Mira N: 888ng Mira C: 888ng Brazz N: 993ng...7 KB (0 words) - 19:20, 26 September 2017
- Ligation Reactions Mira N + V24 Mira C + V24 Brazz N + V24 Brazz C + V24 Control diH2O 7 1 6 4 12 T4 DNA...6 KB (0 words) - 19:13, 26 September 2017
- page Previous entry Next entry Picked colonies from Mira/Brazz StrepII STOP constructs Only Mira N and Brazz C had colonies, 2 mL cultures were started...6 KB (0 words) - 19:18, 26 September 2017
- for 10:10 7= 4°C forever Miniprep of Mira/Brazz+StrepII+STOP in V0120 Gel extraction of J45017: Ladder Mira N N/S Mira C N/S Brazz N N/S Brazz C N/S J45004...6 KB (0 words) - 19:23, 26 September 2017
- microfluidic channel was created or not. [1] Marius, L.; Hofman, V.; Long-Mira E. Circulating Tumor Cells Allow Early Diagnosis of Lung Cancer in Patients...10 KB (1,520 words) - 21:28, 9 May 2017
- NIH/NIGMS Maximizing Investigators’ Research Award for Early Stage Investigators (MIRA) (R35) 2019 Collaborative Science Award, American Heart Association 2019...2 KB (217 words) - 21:27, 20 October 2019
- to Barnase 1 from miniprep onward), LacIN1, and Gal2. Did minipreps of the Mira/Brazz + Strep constructs. All constructs had failed to ligate. Ran PCR of...3 KB (0 words) - 19:17, 26 September 2017
- C-Terminus 2 x B21 S/P + Brazz X/P - N-Terminus 2 x B21 E/X + Mira E/S - C-Terminus 2 x B21 S/P + Mira X/P - N-Terminus 2 x B15 Plate #1 2 x B15 Plate #2 Cultures...9 KB (0 words) - 19:08, 26 September 2017
- notes are not clear. 3/7/2023--Neydis 3/14/2023--NO Group Meeting (Megan--MIRA review) 3/21/2023--Timothy 3/28/2023--Eli 4/4/2023--Renee will take for biosafety...13 KB (2,409 words) - 07:42, 24 June 2024
- Mar;40(3):346-50. DOI:10.1038/ng.77 | PubMed ID:18278046 | HubMed [wittkopp2008] Mira MT, Alcaïs A, Nguyen VT, Moraes MO, Di Flumeri C, Vu HT, Mai CP, Nguyen TH...3 KB (11 words) - 11:42, 24 December 2008
