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- Knight:Miniprep low copy plasmids Alm:Agarose gel electrophoresis Knight:DNA ligation using T4 DNA ligase NanoBio: PCR Sacks:RAD-seq Duffy:LIC Cloning Weiss...10 KB (12 words) - 18:01, 31 August 2017
- Signaling Box Chassis/System - BC Box 1 MG1655(seq) CC4249 PAM162 W3110(λ857S7) LE392 supF You can copy the below code if you want to make a 9X9 Freezer...1 KB (66 words) - 14:16, 30 June 2006
- E. coli genotypes (section MG1655 seqA-eYFP)another copy of lacI elsewhere. This result is somewhat confirmed by the fact that a lacI regulated promoter driving expression of YFP on a medium copy vector...75 KB (13,035 words) - 01:49, 15 February 2023
- biobrick assembly DNA blot (Southern) Polyacrylamide gel electrophoresis DNA Seq Prep Cloning Protocol DNA Quantification Rapid plant gDNA extraction Wwwblast...395 bytes (741 words) - 11:06, 9 October 2007
- first_fasta_DNA.seq second_fasta_DNA.seq dotter first_fasta_AA.seq second_fasta_AA.seq dotter first_fasta_DNA.seq second_fasta_AA.seq Note that the order...56 KB (6,022 words) - 13:26, 21 November 2012
- IGVDxDQ 3 June 2007 N15 cloning vectors, plasmids: AF064539 EF583812 pJAZZ-OC. Copies 24802 (telomere site) - 35005 [MCS, resistance] 23696 - 24802 (telomere...6 KB (913 words) - 20:42, 17 November 2007
- even in model systems, like epigenetic modifications, small RNA regulation, copy number variation, allele specific expression, alternative splicing or antisense...6 KB (786 words) - 07:58, 17 December 2011
- bp annealing region on the fragment ends adjacent to the ligation junction Copy and paste the BsaI sites and 5’ tails on the 5' end of your sequence. An...5 KB (797 words) - 13:27, 8 February 2024
- sequence verification, and DNA Star software SeqBuilder Primer Select EditSeq SeqMan Create sequence file Copy and paste expected sequence from PlasmoDB into...2 KB (194 words) - 11:38, 18 February 2014
- raw_translated_seqs = [] for seq in trimmed_seqs: translated_seq = Seq.translate(seq.replace('-','')) raw_translated_seqs.append(translated_seq) # Create input...17 KB (2,585 words) - 03:01, 27 February 2007
- have been generating and providing cDNA clones, i.e. reverse transcribed copies of mRNAs. Due to the academic or academic+commercial backbone of these enterprises...4 KB (512 words) - 03:12, 12 February 2008
- with Macrogen: Go to http://orders.macrogenusa.com Set up an account Use SynSeq Facility, LTL 027 Department of Molecular Biology Princeton University Princeton...3 KB (382 words) - 15:55, 8 September 2012
- mean the same thing. So I use them interchangeably. Please refrain from just copy-pasting commands from this tutorial into your command line! I strongly believe...38 KB (5,351 words) - 11:21, 28 January 2018
- # (6) seq_obj = Seq.Seq(seq,IUPAC.ambiguous_dna) seq = seq_obj.reverse_complement().tostring() # append the gene sequence self.genes.append(seq) # (7)...47 KB (6,829 words) - 23:04, 2 December 2008
- bed-formatted list: perl extract_bed_seqs.pl list_of_seqs.bed fasta_to_extract outfile_tag_name Writes output to a file named list_of_seqs.bed_tag.fa Generate predicted...32 KB (4,368 words) - 15:10, 8 June 2023
- rearrangements seq_copy = alignment.get_all_seqs() ref_seq = seq_copy.pop(0) # pop out reference sequence (the first one) print "Comparing against", ref_seq.description...15 KB (1,425 words) - 23:20, 19 February 2007
- plasmid was placed backwards. Will repeat KAH87-MV10 once I have a better copy....2 KB (0 words) - 00:57, 27 September 2017
- entry Next entry Repeating last week's experiment with K562 and a working copy of the KAH87-MV10 plasmid. See the Haynes Lab transfection protocol for details...2 KB (0 words) - 00:57, 27 September 2017
- RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Main project page Previous entry Next entry When troubleshooting the transfection of K562 cells...2 KB (0 words) - 00:54, 27 September 2017
- Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Genes/2012/07/23 (section Filter the RefSeq List)trial and error I was finally able to provide a "cleaned up" RefSeq list The problem with RefSeq is that a gene can have multiple mRNA IDs assigned to the same...2 KB (0 words) - 21:46, 26 September 2017
