User contributions for Heather M. Jensen
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14 August 2009
- 10:0510:05, 14 August 2009 diff hist −2,853 NanoBio: Isolation of Periplasmic Fraction →Isolation of Periplasmic Fraction current
10 August 2009
- 14:1514:15, 10 August 2009 diff hist −43 NanoBio: Isolation of Periplasmic Fraction →Spectral measurements of c-cytochromes
- 14:1514:15, 10 August 2009 diff hist −3 NanoBio: Isolation of Periplasmic Fraction →Isolation of Periplasmic Fraction
- 14:1414:14, 10 August 2009 diff hist +2,623 NanoBio: Isolation of Periplasmic Fraction →Isolation of Periplasmic Fraction
- 13:5213:52, 10 August 2009 diff hist −54 NanoBio: Knockout/in →Protocol current
- 13:5113:51, 10 August 2009 diff hist +45 NanoBio: Knockout/in →Introduction
- 13:5113:51, 10 August 2009 diff hist +119 NanoBio: Knockout/in →Protocol
- 13:5013:50, 10 August 2009 diff hist +49 NanoBio: Knockout/in →Protocol
- 13:4613:46, 10 August 2009 diff hist +533 NanoBio: Knockout/in →Protocol
- 13:4413:44, 10 August 2009 diff hist +1,999 NanoBio: Knockout/in →Protocol
- 13:3213:32, 10 August 2009 diff hist +546 NanoBio: Knockout/in →Protocol
- 13:1713:17, 10 August 2009 diff hist +830 NanoBio: Knockout/in →Primer Design
- 13:0313:03, 10 August 2009 diff hist +429 NanoBio: Knockout/in →Knockout/in
- 12:5712:57, 10 August 2009 diff hist +979 N NanoBio: Knockout/in New page: ==Knockout/in== For an example of this, see Heather's notebook C028 (notebook C pg28) The best way to do a knockin is to make the linear fragment of DNA for integration into the genome w...
- 12:5512:55, 10 August 2009 diff hist 0 N File:SOE PCR.jpg No edit summary current
- 12:4212:42, 10 August 2009 diff hist +38 NanoBio:Protocols →Gene deletion with λ-red
- 12:4012:40, 10 August 2009 diff hist +44 NanoBio: AgaroseGels →Imaging your gel current
- 12:4012:40, 10 August 2009 diff hist +44 NanoBio: AgaroseGels →Ingredients
- 12:3912:39, 10 August 2009 diff hist +417 NanoBio: AgaroseGels →Imaging your gel
- 10:0910:09, 10 August 2009 diff hist +1,190 NanoBio: AgaroseGels →Procedure
- 09:5209:52, 10 August 2009 diff hist +238 NanoBio: AgaroseGels →Procedure
9 August 2009
- 22:4722:47, 9 August 2009 diff hist +276 N NanoBio: Isolation of Periplasmic Fraction New page: ==Isolation of Periplasmic Fraction== Protocol courtesy of Yuri Londer, NEB Make sure these chemicals are available: # # # # # Cell growth # Fractionation # *'''[[User:Heather M. Jens...
- 22:4422:44, 9 August 2009 diff hist +82 NanoBio:Protocols →Protein Expression & Purification
- 22:4222:42, 9 August 2009 diff hist +569 NanoBio: Protein Gels →Heme Stain current
15 June 2009
- 11:2111:21, 15 June 2009 diff hist −3 NanoBio: Prep for Electrocompetent cells →Large-Scale prep current
- 11:1711:17, 15 June 2009 diff hist +241 NanoBio: Prep for Electrocompetent cells →Large-Scale prep
12 June 2009
- 16:4116:41, 12 June 2009 diff hist −2 NanoBio: Protocol for gene knockout →. Day 1
- 16:4116:41, 12 June 2009 diff hist +2 NanoBio: Protocol for gene knockout →Day 1
- 15:4715:47, 12 June 2009 diff hist +7 NanoBio: Prep for Electrocompetent cells →Small-scale prep
- 15:4715:47, 12 June 2009 diff hist +48 NanoBio: Bacterial Transformation using Electroporation →Simultaneous Transformation using Electroporation current
- 15:4615:46, 12 June 2009 diff hist +47 NanoBio: Protocol for gene knockout →Colony PCR for scar
- 15:4415:44, 12 June 2009 diff hist +1,800 NanoBio: Prep for Electrocompetent cells →Large-Scale prep
- 15:2915:29, 12 June 2009 diff hist −90 NanoBio: Prep for Electrocompetent cells →Small-scale prep
- 15:2715:27, 12 June 2009 diff hist +36 NanoBio: Prep for Electrocompetent cells No edit summary
- 15:2615:26, 12 June 2009 diff hist −19 NanoBio: Prep for Electrocompetent cells →Large-scale prep
- 15:2615:26, 12 June 2009 diff hist −18 NanoBio: Prep for Electrocompetent cells →Small-scale prep
- 15:2515:25, 12 June 2009 diff hist +1,430 NanoBio: Prep for Electrocompetent cells →Small-scale prep
- 15:2315:23, 12 June 2009 diff hist +163 N NanoBio: Prep for Electrocompetent cells New page: =Small-scale prep= =Large-scale prep= *'''~~~~''': Return to Protocols.
- 15:2215:22, 12 June 2009 diff hist +80 NanoBio:Protocols →Sub-cloning
- 15:2115:21, 12 June 2009 diff hist −1,369 NanoBio: Bacterial Transformation using Electroporation →Simultaneous Transformation using Electroporation
- 15:1715:17, 12 June 2009 diff hist +1 NanoBio: Protocol for gene knockout →Colony PCR for scar
- 15:1715:17, 12 June 2009 diff hist +357 NanoBio: Protocol for gene knockout →Colony PCR for scar
- 15:1315:13, 12 June 2009 diff hist +45 NanoBio: Protocol for gene knockout →Verify deletion with colony PCR
- 15:1215:12, 12 June 2009 diff hist +12 NanoBio: Protocol for gene knockout →PCR verify deletion
- 15:1115:11, 12 June 2009 diff hist −2 NanoBio: Protocol for gene knockout →Glycerol stocks to keep
- 15:1115:11, 12 June 2009 diff hist +485 NanoBio: Protocol for gene knockout →Remove antibiotic resistance with pCP20
- 13:3113:31, 12 June 2009 diff hist +6 NanoBio: Protocol for gene knockout →Remove antibiotic resistance with pCP20
- 13:3013:30, 12 June 2009 diff hist 0 NanoBio: Protocol for gene knockout →PCR verify deletion
- 13:3013:30, 12 June 2009 diff hist +92 NanoBio: Protocol for gene knockout →PCR verify deletion
- 13:2913:29, 12 June 2009 diff hist +6 NanoBio: Protocol for gene knockout →Electroporate linear PCR product into electrocompetent cells
