Rules
- Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common.
- Only common enzymes may have backup, which are stored in main -20C.
- Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI
- The enzymes not listed are in the non-common enzyme cooler.
- If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested.
- When the working stock has been used up, replace with a new tube from the backup cooler and write the enzyme on the whiteboard.
- Backup ligases and CIP are also in the backup cooler in main -20C.
Restriction Enzymes in Stocks
| Enzyme | Regular | HF | Regular Backup | HF Backup
|
| AflII | Y | | |
|
| AgeI | Y | Y | | Y
|
| AleI | Y | | Y |
|
| ApaI | Y | | YY |
|
| AscI | Y | | |
|
| AsiSI | Y | | Y |
|
| AvrII | Y | | Y |
|
| BamHI | Y | Y | | Y
|
| BglII | Y | | |
|
| BsaI | | Y | |
|
| BsrGI | Y | | |
|
| BstBI | Y | | |
|
| BstXI | Y | | |
|
| Bsu36I | Y | | |
|
| ClaI | Y | | Y |
|
| DpnI | Y | | Y |
|
| EcoRI | | Y | | Y
|
| EcoRV | | Y | | Y
|
| FseI | Jay's -80 | | |
|
| HindIII | Y | Y | Y |
|
| KpnI | Y | Y | | Y
|
| MfeI | | Y | | Y
|
| NdeI | Y | | |
|
| NheI | Y | Y | |
|
| NotI | Y | Y | | Y
|
| NruI | Y | | |
|
| PacI | Y | | |
|
| PmeI | Y | | |
|
| PstI | Y | | |
|
| PvuII | | Y | |
|
| SacI | | Y | | Y
|
| SacII | Y | | Y |
|
| SalI | Y | Y | | Y
|
| SapI | Y | | |
|
| SbfI | | Y | |
|
| SpeI | Y | | Y |
|
| SphI | | Y | |
|
| SwaI | Y | | |
|
| XbaI | Y | | Y |
|
| XhoI | Y | | Y |
|
| XmaI | Y | | |
|
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