Shreffler:Notebook/Staff Meetings/2010/08/03

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*Wayne G. Shreffler 10:49, 3 August 2010 (EDT):

Agenda Items

  • EoE
    • EDN ELISAs
      • Sarita has list from Marcella and will order a kit or two to evaluate
    • Component proposal -- WS still needs to work on
  • Basophil
    • Review data from last week (Sarita, Alex sens #7,8)
      • Sarita's experiment low baso counts, but good Ag response
      • evidence of pre-existing Derp2 sensitization; however, clear evidence of stronger degran with 50:50 sens than 99:1 or 1:99
      • Next experiments
        • Repeat with buffy's this week, consider lengthening stripping time?
    • Caitlin paper? -- Bert has looked?
      • no discussion today
  • Allergen Adjuvant
    • Review outcome of meeting w/ S. Carr
      • Willing to perform digest/ MS and see
    • Review most recent PCR data on dose response to fraction 3
      • strong evidence of correlation with prominent band on dilution experiment -- very nice response, much stronger in Fr3 than whole CPE as well
    • F/U with Carr
      • Bert will take gels over THIS week
    • Forski's?
      • decent number of CD45+DR+CD1a+, but no evidence of Aledflour enhancement; staining/ flow not optimal
  • PM Baso
    • nothing new
  • JAX
    • quote for peptide arrays
      • WS has to do
    • train Stephanie for project role
  • U19
    • Discuss involvement with Bert



Other Discussion

  • Isabelle will see bakery owner on Thu; working on dietary guideline doc, will meet w/ her tomorrow morning



Things To Do

  1. Investigate EDN assay options/ request samples w/ standards (Sarita)
  2. Write up component proposal (Marcella and Wayne)
  3. obtain quote for peptide arrays
  4. send Bert U19 slides
  5. send Isabelle Dowling the dining services director name/ contact





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