Agenda Items
Alex's work
- JAX
- scanner works
- need to make a plan to work with Kunal
- gating strategy for template does not exclude DR currently
- Alex will review protocol to come up with total number of basophil samples that require staining and request a quote from BD for cocktail (minus the CRTH2).
- look into buying lot of CRTH2 from Miltenyi
- PM
- Alex getting data organized/ analyzed
- R03
- Reviewed data from 5 experiments (3/5 look like measurable DHR response)
- tentatively looks as though DHR is suppressed by EDTA
- Alex to export data from FJ to tables to complete analysis
- Carbo
- made a new template using CRTH2 -- suggests that there may be substantial DR expression!
Action Items
- Wayne will talk to Ben about using qPCR more regularly (done)
- Bert will compare LPS response
- run fraction screening experiment on Ben's machine
- repeat fraction screening experiment with pooled fractions
- get larger column for bigger run
- contact Steve about using HPLC (not done)
Other Discussion
Things To Do
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ShreffLab Staff Meetings
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