Shreffler:Notebook/Staff Meetings/2010/03/23

ShreffLab Staff Meetings

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*Wayne G. Shreffler 15:58, 23 March 2010 (EDT): Met w/ Sarita re: project in the summer We discussed aims 1 and 2 of her basophil project Aim 1: Derp2-IgE complex fate. Hypothesis: in tact Derp2 is sequestered by basophils either on surface or intracellular site without degradation in a manner that could subsequently stimulate B cells Use mAbs and rDerp2 to follow antigen fate following cross-linking Bert and Alex reviewed spending external hard drive freezer Calls with JAX and Hopkins JAX Thu afternoon Samples expected from JHU -- call next week Alex will send Mouse urine extract to MJ R03 NAC experiment pH of 30 uM NAC is very low -- Alex will figure out how to neutralize and measure pH of all dilutions to determine what data we can retain CPE/ DC Protein ID Things To Do