SBB10Ntbk-JennaKelleher2

From OpenWetWare

Jump to: navigation, search

Labwork

Prepared sbb28 and sbb29 for PCR after creating a master mix with Jeni Lee
sbb28 in 2k55 and sbb29 in 55

Updated Constuction Files

Biobricking of 099 rep protein basic part sbb29

PCR repo99-F and JG002-R on pEC52 gene             (978 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144                           (EcoRI/BamHI, 910+2472, L)
Product is pBca9523-sbb29	{rbs.repO99!} 
----------------------------
repo99-F   Cloning of repo99   
ccaaaGAATTCatgAGATCTcctggaaggaatcgtaacac
JG002-R   Cloning of  O99 rep Gene   
CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc

Biobricking of CA42 rep gene basic part sbb28

PCR repCA42-F and KRM002 on pEC22-CA42 gene      (1496 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144                           (EcoRI/BamHI, 910+2472, L)
Product is pBca9523-sbb28     {5'UTR.repCA42!} 
----------------------------
repCA42-F   Cloning of repCA42   
ccaaaGAATTCatgAGATCTcagctgaagtgaccggattag
KRM002   Reverse oligo for cloning of rbs.repCA42!    
ctgatGGATCCctattttccgcttttccag

Biobricking of pre-pro sequence basic part sbb42

Eco/Bam transfer pBjh1601AC-Bjh1723
Sub into pBjk2741-Bca1144            (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-Bjh1723  	{rbs_pelB>}
Personal tools