SBB10Ntbk-JennaKelleher2
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Labwork
Prepared sbb28 and sbb29 for PCR after creating a master mix with Jeni Lee
sbb28 in 2k55 and sbb29 in 55
Updated Constuction Files
Biobricking of 099 rep protein basic part sbb29
PCR repo99-F and JG002-R on pEC52 gene (978 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144 (EcoRI/BamHI, 910+2472, L) Product is pBca9523-sbb29 {rbs.repO99!} ---------------------------- repo99-F Cloning of repo99 ccaaaGAATTCatgAGATCTcctggaaggaatcgtaacac JG002-R Cloning of O99 rep Gene CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc
Biobricking of CA42 rep gene basic part sbb28
PCR repCA42-F and KRM002 on pEC22-CA42 gene (1496 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144 (EcoRI/BamHI, 910+2472, L) Product is pBca9523-sbb28 {5'UTR.repCA42!} ---------------------------- repCA42-F Cloning of repCA42 ccaaaGAATTCatgAGATCTcagctgaagtgaccggattag KRM002 Reverse oligo for cloning of rbs.repCA42! ctgatGGATCCctattttccgcttttccag
Biobricking of pre-pro sequence basic part sbb42
Eco/Bam transfer pBjh1601AC-Bjh1723 Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-Bjh1723 {rbs_pelB>}