ONP

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o-nitrophenol

  • Beta-galactosidase cleaves ONPG to produce ONP and galactose.
  • The molar extinction coefficient is reported to be either 4500 M-1cm-1 [1] or 4900 M-1cm-1 [2] (not original references, better references would be preferable).
  • Slightly soluble in water (0.21 g/100ml or 2.1mg/mL)

[edit] References

  1. Skoneczny M and Rytka J. Oxygen and haem regulate the synthesis of peroxisomal proteins: catalase A, acyl-CoA oxidase and Pex1p in the yeast Saccharomyces cerevisiae; the regulation of these proteins by oxygen is not mediated by haem. Biochem J 2000 Aug 15; 350 Pt 1 313-9. pmid:10926859. PubMed HubMed [Skoneczny-BiochemJ-2000]
  2. Onishi N and Tanaka T. Purification and properties of a novel thermostable galacto-oligosaccharide-producing beta-galactosidase from Sterigmatomyces elviae CBS8119. Appl Environ Microbiol 1995 Nov; 61(11) 4026-30. pmid:8526517. PubMed HubMed [Onishi-ApplEnvironMicrobiol-1995]
  3. Chemical information [o-nitrophenol]
All Medline abstracts: PubMed HubMed
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