Beta-galactosidase

From OpenWetWare

Some interesting facts [1]

• A tetramer of 4 identical subunits
• Each subunit is 120kD.
• Active only as a tetramer.
• Mutations in some of the codons of the N-terminal 60aa or C-terminal 100aa results in an inactive, dimeric β-galactosidase. [2]
• If the above sequences are deleted, the missing protein fragment can be replaced by the corresponding peptide. This is called intracistronic alpha or omega complementation respectively.
• Its N-terminal 23 residues can be replaced by any amino acid residues without affecting the enzymatic activity.
• A mutant with an internal deletion of codons 21-41 of the lacZ gene does not produce any active β-galactosidase.
• A mutant with a deletion of everything past residue 60 (i.e. it expresses only the first 60 N-terminal amino acids) does not produce any active β-galactosidase.
• You can measure lacZ activity using flow cytometry. See A flow cytometric study of stationary phase gene expression in E.coli using lacZ reporter gene fusion
  • This paper uses C₁₂FDG from Invitrogen. However, FDG might be a better substrate in gram-negative bacteria like Escherichia coli [3]
• Another fluorogenic substrate is 4-methylumbelliferyl β-D-galactopyranoside (MUG) which works in bacteria, yeast, and mammalian cells (without requiring permeabilization/lysis). [4]
• Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype [5]. (If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.)

Protocols

• beta-galactosidase assay
• LacZ staining of cells

See also

• X-Gal

Reference

1. Benno Muller-Hill. The Lac Operon. Walter de Gruyter. isbn:3-11-014830-7. [Muller-Hill-1996]
2. isbn:0317118099. [Beckwith-1970]
3. Plovins A, Alvarez AM, Ibañez M, Molina M, and Nombela C. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl Environ Microbiol 1994 Dec; 60(12) 4638-41. pmid:7811104. PubMed HubMed [Plovins-ApplEnvironMicrobio-1994]
4. Vidal-Aroca F, Giannattasio M, Brunelli E, Vezzoli A, Plevani P, Muzi-Falconi M, and Bertoni G. One-step high-throughput assay for quantitative detection of beta-galactosidase activity in intact gram-negative bacteria, yeast, and mammalian cells. Biotechniques 2006 Apr; 40(4) 433-4, 436, 438 passim. pmid:16629389. PubMed HubMed [Vidal-Aroca-2006]
5. Zamenhof PJ and Villarejo M. Construction and properties of Escherichia coli strains exhibiting -complementation of -galactosidase fragments in vivo. J Bacteriol 1972 Apr; 110(1) 171-8. pmid:4552986. PubMed HubMed [Zamenhof-JBacteriol-1972]
6. Ullmann A. Complementation in beta-galactosidase: from protein structure to genetic engineering. Bioessays 1992 Mar; 14(3) 201-5. doi:10.1002/bies.950140311 pmid:1345751. PubMed HubMed [Ullmann-Bioessays-1992]
7. [by] Jeffrey H. Miller. Experiments in molecular genetics. [Cold Spring Harbor, N.Y.] Cold Spring Harbor Laboratory, 1972. isbn:0879691069. [Miller-1972]

   original β-galactosidase assay by Miller

8. Thibodeau SA, Fang R, and Joung JK. High-throughput beta-galactosidase assay for bacterial cell-based reporter systems. Biotechniques 2004 Mar; 36(3) 410-5. pmid:15038156. PubMed HubMed [Thibodeau-Biotechniques-2004]
9. http://www.worthington-biochem.com/BG/default.html

   [WorthingtonBiochem]

10. Serebriiskii IG and Golemis EA. Uses of lacZ to study gene function: evaluation of beta-galactosidase assays employed in the yeast two-hybrid system. Anal Biochem 2000 Oct 1; 285(1) 1-15. doi:10.1006/abio.2000.4672 pmid:10998258. PubMed HubMed [Serebriiskii-AnalBiochem-2000]