Maloof Lab:loading dye

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Loading dyes and DNA markers are stored in a gel room refrigerator. 6x Xylene Cyanol Loading Buffer for Electrophoresis This buffer will have only the light blue than runs slower on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary. 100mg Xylene-cyanol 6g of Ficoll (Type 400) Add water to 40 ml Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. 6x Bromophenol Blue Loading Buffer for Electrophoresis This buffer will have only the dark blue than runs faster on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary. 100mg Bromophenol Blue 6g of Ficoll (Type 400) Add water to 40 ml Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. Another protocol (Liron Shalom) is 25mg Bromophenol Blue 3 ml of glycerol Add water to 10 ml Loading Buffer with GelRed for Electrophoresis To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer described above. This protocol will make 5ml of 6x solution. Adjust to your needs if necessary. 5 ml Loading Buffer (Xylene-cyanol or Bromophenol Blue) 15 ul of GelRed (10000x in water, cat.41003 Biotium) 1 Kb Ladder for Electrophoresis Use the ThermoFisher GeneRuler 1kb ladder or GeneRuler 1kb plus from the stock. Mix: 100µl (1 tube) DNA Ladder 100µl 6X Loading Dye Solution 400µl Deionized water Good to go. Load 3 to 5 µl.