IGEM:University of Chicago/2009/Notebook/Organophosphate Hydrolase/2009/10/06

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Mutagenesis Results

  • Picked up plates from yesterday
Plate 1:
    10μL  - 9 colonies
    500μL - ~ 800 colonies
Plate 2:
    10μL  - 15 colonies
    500μL - ~ 500 colonies
Plate 3:
    10μL  - 2 colonies
    500μL - 30 colonies
Plate 4:
    10μL  - 1 colony
    500μL - 60 colonies
Plate 316:
    No colonies
Plate 314:
    No colonies
  • Created 4 starter cultures per number
  • Finished accuprime pfx reactions - dpnI digest, transform



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