- For the first step in paraoxon degradation, we will express organophosphate hydrolase on the yeast cell-wall surface using the Aga2 cell-surface display system. Originally designed for antibody display, this system displays the protein of interest flanked on the N-terminus by Aga2, the disulfide of which binds the myristylated surface membrane protein Aga1. Aga1 and Aga2 are cell-surface glycoproteins which facilitate mating type-specific agglutination of Saccharomyces cerevisiae a and alpha cells. Proper surface expression is dependent on Aga1 expression--Aga2 tagged proteins would otherwise be completed secreted outside the cell wall.
- Team meeting today at 3:30 (July 31)