IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct/TestingValidation
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| Super Parts | Prey Construct | ||
|---|---|---|---|
| Actual Part |  T9002: Test Sensing Prey Construct | ||
| Sub Parts | F2620 | intermediate_part | intermediate_part |
Motivations
- With this testing part, we want to characterize the sensitivity of the prey cell towards the prey molecule (AHL)
- By testing the part firstly with known AHL concentrations, a transfer curve can be established
- By referring to the transfer function, this part will serve as an assay for AHL concentrations, which allows us to quantize different AHL concentrations
- The expression of GFP and thus the level of fluorescence is dependent on the concentration of AHL
Protocol
Results
Testing Results (processed data) for T9002:
- 02/09/2006
- 01/09/2006
- 31/08/2006
- 30/08/2006
- 28/08/2006
- 27/08/2006
- 25/08/2006
- 24/08/2006
- 23/08/2006
- 22/08/2006 plate 1 and 22/08/2006 plate 2 (96-well plate was left in shaker overnight)
Parameter extraction
- Demonstrate how you are extracting the paramters you are interested in from experimental data.
- Give the results.
