IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct/Design

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Super Parts Prey Construct
Actual Part
T9002: Test Sensing Prey Construct
Sub Parts F2620 intermediate_part intermediate_part


Registry

  • <bbpart>BBa_T9002</bbpart>
image:T9002.JPG

Design choices

  • This part is already available in the Registry
  • It has the following properties that we need in order to characterize the Prey Sensing Part:
    • The part responds to AHL levels, via activation of pLuxR
    • From this, we can calibrate a AHL assay based on fluorescence
    • LuxR is under the control of pTet, allowing different levels of LuxR to be achieved
  • AHL Levels:
    • The model is sensitive to initial AHL levels
    • As AHL input increase, GFP output increases until saturation
    • The relationship between AHL level and GFP output is described by the equation above
    • The exact relationship can be calculated from this assay


INPUTS biological component comments
Prey molecule AHL Homoserine lactone synthesized by the LuxI gene <bbpart>BBa_C0061</bbpart>
OUTPUTS biological component(s) comments
Pops GFP Green Fluorescent Protein <bbpart>BBa_E0040</bbpart>

Open issues

A potential issue with the experiment is regarding using pTet as the promoter for LuxR expression. It may be necessary to add a gene for TetR expression to ensure control over this promoter.

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