IGEM:Cambridge/2008/Notebook/Voltage/2008/08/27

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osmY RBS Primers containing RBS for osmY Biobrick were received PCR was performed from biobrick J45250 Ligated into dephosphorylated vector backbone Colonies transformed Restriction Digest & Ligation Test Various combinations of restriction digests & T4 phage genome ligation test. All results positive :-)