IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/08/19

iGEM Project name 1

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cPCR preparaion: summary innoculated and made spot plate of constructs from Aug 19 The constructs were: pSB1C3 + LoxR pSB1A3 + LoxR pSB1C3 + Cre-LVA pSB1A3 + Cre-LVA The innoculation performed in triplicate using standard UBC iGEM protocol picked 3 colonies from each plate, each colony into 5mL LB with appropriate antibiotic (Ampicillin for pSB1A3 constructs and chloramphenicol for psB1C3 constructs) to test that the antibiotics are effective, also had negative controls: innoculated a pSB1A3 colony into LB-Chlor and a pSB1C3 colony into LB-Amp overnight innoculation